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  • rnaseq
    Junior Member
    • Dec 2009
    • 7
    #1

    fold change for genes with 0 reads

    I am comparing Illumina RNA-seq data sets for differentially expressed genes. A significant number of genes have 0 reads in one of the two conditions. What is the best way to include these genes? Is it acceptable to subsitute the 0 in the numerator or denominator with another number to get an approximate idea of the fold change?

    Thanks!
    Tags: None
    • malachig
      Senior Member
      • Aug 2010
      • 117
      #2
      This appears to be a cross post. FAQ says not to do this...

      In case anyone lands on this one, the post that has generated discussion is here.

        Comment

        • ECO
          --Site Admin--
          • Oct 2007
          • 1360
          #3
          Closed. Thanks malachig.

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