I am new to the Illumina NGS system and am trying to begin analyzing some data. What are the file formats that are generated by the Illumina machine and what are the best formats to convert them into for subsequent analysis? For example, are they generated in an Illumina specific file, like .ill or something, and then to start analysis I need to convert them to .fasta or .seq or .txt ?
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You can find the details of illumina's output files format in the casava documentation. As for the posterior conversion to other formats, most of the downstream tools come with scripts to convert from illumina(and other platforms) to the format used by the tool (typically fastq for mapping).-drd
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by SEQadmin2
CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...-
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