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  • mccullou
    Member
    • Oct 2008
    • 13

    Fluidigm can help with sequencing?

    I have read in various places (mostly their literature) that Fluidigm "eliminates costly titration steps" in next generation sequencing. I am unclear exactly how their technology really helps. Are they just using the SlingShot to get a better estimate of the copy number for titration? If this is the case, it hardly seems like a time or cost savings. http://www.fluidigm.com/applications/slingshot.html

    Having not run a clonal/ePCR next gen sequencing reaction I don't know to what degree this is a problem and if this SlingShot would help. Is there anyone out there that does do this titration regularly and feels this would help or is this just another marketing gimmick?

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  • SEQadmin2
    Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by SEQadmin2


    I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

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  • SEQadmin2
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    The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
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