Hi, at the risk of exposing my ignorance, a question: can someone point me to a reference that established the use of 30X coverage as a goal in sequencing? Where does this number come from? Why not 10X or 50X?
-
-
Usually related to the papers that used SNP arrays and NGS and tested the level of sequencing needed to call all heterozygote SNPs detected on the array by NGS or sanger sequencing. The first good example I remember is the Ventor diploid genome paper in Plos One but a number of similar examples are available. Scan the genome papers that also have a SNP array in the materials and methods and check their supplemental tables. But most I remember show diploid concordance >95% is not reached till around 26x or greater coverage
-
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM -
Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.
We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing...
Comment