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  • Michael L. Altshuler
    Member
    • Nov 2007
    • 14

    Terms defining sequencing accuracy

    Hi colleagues,

    I am aware that with respect to accuracy the new generation methods can lag behind the Sanger’s.

    The requirement of repeated sequencing of the same nucleotide stretch can apparently be expressed in different ways. What is the difference between “per base read accuracy” and “consensus accuracy”? Also between “sequencing coverage” and “bases covered %”. How these terms are connected with “sample depth”?

    I have been unable to find satisfactory answers to these questions in Wikipedia. Is there any other free educational resource addressing this theme?

    Thank you in advance,
    Michael

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  • GATTACAT
    Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by GATTACAT
    Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
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  • SEQadmin2
    Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by SEQadmin2


    I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

    Here are nine questions we think about, in roughly the order they matter, before...
    06-18-2026, 07:11 AM

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