Did you do the Wig to bigWig conversion. This is recommended for large datasets. A tool to convert the file can be downloaded here:
hope that helps...
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Thank you! But I still have a problem: First, I uploaded the data for the X-chromosome for my sample. When finished the page "manage custom tracks" appears. I marked the data with an x and clicked on "add custom tracks" and submitted the data for the X-chromosome for my negative control. But after a while this error pops up: needMem: trying to allocate 528709463 bytes (limit: 500000000). What can I do?Leave a comment:
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Just upload both of them to UCSC (submit one file each time), then you can see both of them on Genome Browser.Leave a comment:
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Adding NGS data to the UCSC genome browser
Hi everybody,
I´m a phd student from Germany analyzing the functional mechanism of a transcription factor and trying to find out new target genes. We used ChIP-seq analysis to identify the new target genes. Now, I´m trying to work with and understand the NGS data: using the UCSC genome browser I would like to see the differences between my sample and the negative control. By adding new cumstom tracks I can upload one set of data in wig format (=one chromsome).
Can anybody tell me how I am able to upload TWO sets of data in wig format to the UCSC genome browser so I can se the differences between the sample and control???
Thanks a lot!
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM
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