Adding NGS data to the UCSC genome browser

[ulz_peter]

Did you do the Wig to bigWig conversion. This is recommended for large datasets. A tool to convert the file can be downloaded here:

http://hgdownload.cse.ucsc.edu/admin/exe/

hope that helps...

[aer]

Thank you! But I still have a problem: First, I uploaded the data for the X-chromosome for my sample. When finished the page "manage custom tracks" appears. I marked the data with an x and clicked on "add custom tracks" and submitted the data for the X-chromosome for my negative control. But after a while this error pops up: needMem: trying to allocate 528709463 bytes (limit: 500000000). What can I do?

[Gangcai]

Just upload both of them to UCSC (submit one file each time), then you can see both of them on Genome Browser.

[aer]

Hi everybody,
I´m a phd student from Germany analyzing the functional mechanism of a transcription factor and trying to find out new target genes. We used ChIP-seq analysis to identify the new target genes. Now, I´m trying to work with and understand the NGS data: using the UCSC genome browser I would like to see the differences between my sample and the negative control. By adding new cumstom tracks I can upload one set of data in wig format (=one chromsome).
Can anybody tell me how I am able to upload TWO sets of data in wig format to the UCSC genome browser so I can se the differences between the sample and control???
Thanks a lot!