Thanks, very helpful
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Last edited by grimani; 02-19-2020, 11:22 PM. -
To me the data you mentioned are no big reason to worry. They gave you more data for the first sample, but duplicates are certianly higher than expected.
The biases visible for the first 16 nt in the second plot indicate that they use Nextera or other transposase library preps - I do not like this chemistry because it introduces more biases than necessary.
The slightly varying read lengths is very curious - I have no clue how these could have been generated.
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The immune system’s power comes from its genetic diversity, allowing myriad threats to be neutralized through first recognizing foreign antigens. That diversity is also what makes the immune system so difficult to study. Recent advances in sequencing technology and computational biology, however, are giving researchers new tools to understand immune responses and immune-related diseases in greater detail.
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