I have a problem running Blast nt database against my query sequences(fungal assembly). I noticed that BLAST is skipping some of the queries i.e contigs from the assembly. Can anyone please help? How can I make Blast to process all queries? Is it something to do with the CPU time limit? I even tried splitting my assembly file into smaller parts and submit each part separately. It still skips some contigs from the smaller parts too.*
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Are you referring to online blast? Please read about the new default blast limits in place: https://www.biostars.org/p/444732/ You would need to change the e-value most likely. -
No, I am running blast remotely on a computer cluster. Thanks for that valuable information! I changed e-value and blast skips less queries now.Originally posted by GenoMax View PostComment
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CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM
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