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Hi everyone,
I have my transposon mutant library, and I want to visualize the mapped transposon insertions to the reference genome via DNAplotter (Artemis).
I need the plot to be like the attached picture from the literature (where the red lines represent the transposon insertions).
I could not do the same in Artemis because non of the output files from BioTradis pipeline are in EMBL format. I can only visualize BAM and insertion plot as shown in (My_entry). Therefore, could anyone help or suggest how can I convert the files that mapped to the reference genome (e.g., BAM, fq.gz) to EMBL in order to visualize the insertions in DNAplotter.
Thanks in advance,
I have my transposon mutant library, and I want to visualize the mapped transposon insertions to the reference genome via DNAplotter (Artemis).
I need the plot to be like the attached picture from the literature (where the red lines represent the transposon insertions).
I could not do the same in Artemis because non of the output files from BioTradis pipeline are in EMBL format. I can only visualize BAM and insertion plot as shown in (My_entry). Therefore, could anyone help or suggest how can I convert the files that mapped to the reference genome (e.g., BAM, fq.gz) to EMBL in order to visualize the insertions in DNAplotter.
Thanks in advance,