Can any body tell me what is base calling and calling SNPs?
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I think it depends on the context...
But I'd say that "base calling" is the method by which some software looks at the raw data from your instrument, probably some kind of fluorescence, and decides whether there is an A,C,G or T there.
SNP calling involves comparing this info to a reference sequence, and if there is a discrepancy, deciding if the quality is good enough to justify saying "your sample has a variation" -
in most of the mutation detection software tools we need to provide forward and reversce trace files along with the reference sequence files.
What is this forward/reverse trace files and why we use this kind of aproach?Last edited by asankaf; 02-04-2009, 10:28 PM.Comment
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