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  • Smriti
    Member
    • Mar 2011
    • 40
    #1

    Lambda DNA, Methylated from Ecoli

    Hi All,

    Recently I ordered Lambda DNA from Sigma which says "Methylated from Ecoli- partially cleaved by Bcl I, Cla I, Mbo I, Mbo II, taq I and Xba I". Similarly, the Non-methylated version is completely cleaved by these enzymes.

    What type of partial or complete cleavage is this? Is this cleavage within Lambda DNA or some cleavage between Lambda and E coli DNA?

    If this cleavage is within Lambda DNA, then how do we see a single band of 48Kb and not a smear of range of fragments.
    Can anyone explain this?

    Thanks
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