Unconfigured Ad

Collapse
X
Collapse
 
  • Page of 1
  • Filter
  • Time
  • Show
Clear All
new posts
Previous template Next
  • Fiji_surf
    Junior Member
    • Mar 2011
    • 2
    #1

    miRNA rank order

    Hi all,

    I have submitted my total RNA from mouse liver for small RNA sequencing by vendor on the East coast and got the results back. I analyzed the data using CLC Workbench tool and got the ranking of miRNAs that are expressed in liver. Surprisingly, miR-122 (a known top miRNA in liver) came number six...
    I thought that there is something wrong with my analysis and downloaded publicly available data set for mouse liver, analyzed it and got miRNA rank order as it was predicted.
    So, now, that I know that my analysis is fine, I wonder: is there something vendor does to my samples, so that miRNA rank order changes? In other words, how the rank order depends on the operator who performs sequencing procedure?

    Thanks.
    Tags: None
  • Livi81
    Member
    • Apr 2010
    • 22
    #2
    Could it be a question of biology? Is your liver from the same sex, age, strain mouse as the published data set?

    Comment

    Previous template Next

    Latest Articles

    Collapse
    • GATTACAT
      Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing
      by GATTACAT
      Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
      07-01-2026, 11:43 AM
    • SEQadmin2
      Nine Things a Sample Prep Scientist Thinks About Before Sequencing
      by SEQadmin2


      I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

      Here are nine questions we think about, in roughly the order they matter, before...
      06-18-2026, 07:11 AM
    View All

    ad_right_rmr

    Collapse

    News

    Collapse
    Topics Statistics Last Post
    Engineered Protein Motor Takes Its First Steps Along DNA Track by SEQadmin2
    Started by SEQadmin2, Yesterday, 11:05 AM
    0 responses
    7 views
    0 reactions
    		 Last Post SEQadmin2
    by SEQadmin2
    Yesterday, 11:05 AM  
    High-Resolution Sequencing Exposes Hidden Toxoplasma Diversity by SEQadmin2
    Started by SEQadmin2, 07-02-2026, 11:08 AM
    0 responses
    28 views
    0 reactions
    		 Last Post SEQadmin2
    by SEQadmin2
    07-02-2026, 11:08 AM  
    New AI Model Captures Long-Range Genomic Signals to Improve RNA Splice Site Prediction by SEQadmin2
    Started by SEQadmin2, 06-30-2026, 05:37 AM
    0 responses
    27 views
    0 reactions
    		 Last Post SEQadmin2
    by SEQadmin2
    06-30-2026, 05:37 AM  
    Large-Scale Protein Screen Uncovers Hidden Regulators of Alternative Polyadenylation by SEQadmin2
    Started by SEQadmin2, 06-26-2026, 11:10 AM
    0 responses
    27 views
    0 reactions
    		 Last Post SEQadmin2
    by SEQadmin2
    06-26-2026, 11:10 AM  
    View All
    Working...