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  • ttnguyen
    Member
    • Mar 2010
    • 41
    #16
    Originally posted by andreiafonseca View Post
    can you explain me what do you mean by marking PCR duplicate?
    The DNA fragments maybe duplicated due to PCR amplification. It means you may have many reads mapped to the same position. Picard can help with removing duplicate.
    http://picard.sourceforge.net/comman...overview.shtml

    You can find more information about that in this forum as well.

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      • ttnguyen
        Member
        • Mar 2010
        • 41
        #17
        I think that is fine when you chose the -v alignment mode since the QS is good.

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