I have a basic question I can not figure out.
When people do evolution accumulation sequencing of wt vs. a specific mutant (or sequence cancer vrs normal). how do they tell that the effect they see is really mutant-specific. I guess they map the reads to the reffence, got eventually two VCF files but then what?
If you can point me to specific papers that would be great.
Thanks!
When people do evolution accumulation sequencing of wt vs. a specific mutant (or sequence cancer vrs normal). how do they tell that the effect they see is really mutant-specific. I guess they map the reads to the reffence, got eventually two VCF files but then what?
If you can point me to specific papers that would be great.
Thanks!