where can I find a basic but very comprehensive over view and comparison of all DNA sequencing protocols including sanger techniques and next generation sequencing?
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Nowhere to my knowledge, but I'd also be interested in one. I wrote up a short, and not particularly detailed overview of the 3 next-gen sequencing technologies here: http://www.genographia.org/portal/to..._download/file if anyone here is interested in putting together a more comprehensive document I'd be happy to help if I can. -
Go to www.nature.com/naturebiotechnology and search for the October 2008 issue, volume 26, number 10. Or find the paperback journal.
The Focus was on next gen sequencing with comparisons made to Sanger. It was very comprehensive and well written in my view with good illustrations. At the very least, I highly recommend adding this issue to your collection as it will quickly and definately answer many questions you may have.Last edited by seqgirl123; 02-21-2009, 02:53 PM.Comment
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comprehensive reviews
Any idea about a comprehensive review of front end sample enrichment? It seems every time I turn around there's another offering. I'm trying to compile a comprehensive list, since we haven't actually started doing this yet.
NimbleGen, Agilent, Fluidigm, Febit, RainDance....
Thanks
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the sequence enrichment systems list would be very interesting and usefull for my lab. We are actually searching for the most usefull and cheap one for 454 resequencing (for a genotyping program). If anybody has a list / description or info about the previously mentioned ones: NimbleGen, Agilent, Fluidigm, Febit, RainDance....
please, share it w us!Comment
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Also Combimatrix is thinking of using it's arrays for target seq enrichment, we are going to perform the first tests (sequencing with our 454) in the following weeks. Stay tuned !Comment
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This is a good review of sequencing systems, from Sanger to Next-gen to Next-next-gen. Seemed pretty concise and pretty complete (and plenty of pictures ;-)
Wilhelm Ansorge. Next-generation DNA sequencing techniques.
New Biotechnology, Volume 25, Issue 4, April 2009, Pages 195-203.Comment
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CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
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