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Hi,
I'm a Bioinformatics Analyst at the Whitehead. Have been recently introduced to the world of Next-Gen sequencing. One of my projects deals with sequencing host and pathogen transcripts isolated from infected cells.
Questions for the community:
Are there established error or background read distributions that would allow me to attach a p-value to an observed read count?
We are planning to use spike-ins to use observed read-counts to estimate transcript copy number. Are any of you using spike-ins in your sequencing experiments? If so, how are you estimating amount of spike-in to add?
TIA,
Anjan
I'm a Bioinformatics Analyst at the Whitehead. Have been recently introduced to the world of Next-Gen sequencing. One of my projects deals with sequencing host and pathogen transcripts isolated from infected cells.
Questions for the community:
Are there established error or background read distributions that would allow me to attach a p-value to an observed read count?
We are planning to use spike-ins to use observed read-counts to estimate transcript copy number. Are any of you using spike-ins in your sequencing experiments? If so, how are you estimating amount of spike-in to add?
TIA,
Anjan