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  • zmartine
    Junior Member
    • Nov 2009
    • 4

    #1

    Greetings from NIH!

    Hey there. I am an evolutionary geneticist at NIH, working on the comparative genomics of malaria parasites. I'm not new to using next gen sequence information, but I am a very much a beginner at generating it. I am learning about .sam/.bam./.bam.bai/.sorted.bam/.vcf files, as well as shell scripting, as I go along. Every time I Google something I end up here, so it seems like a place I should frequent. Cheers!
    Last edited by zmartine; 02-06-2012, 04:07 PM.

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    Reply to 16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
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  • SEQadmin2
    16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
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    NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...
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