Just thought I'd introduce myself. I'm a postdoc at NHGRI at the NIH, where I'm making the transition from the wet lab to a more computationally-focused approach. I've been employing high-throughput sequencing in my projects for a while, but have only been using command-line-based interactions with the data on a routine basis in the last few months. I've heard good things about SEQanswers, so I'm looking forward to learning from the community's expertise!
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CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM -
Proteomics platforms are evolving rapidly, with advances in mass spectrometry and affinity-based approaches expanding what researchers can detect and at what scale. As the field moves toward deeper proteome coverage and clinical applications, scientists face an increasingly complex landscape of tools. This article will explore how researchers are navigating these choices to find the right platform for their work.
The systematic characterization of the human proteome has...07-20-2026, 11:48 AM