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Hi! I'm Jackie. I am currently studying Cancer Biology and hope to better my understanding and my knowledge about sequence analysis!
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Welcome
Welcome to the forum, Jackie. Here is a recent open access article I thought I would mention in case you may be interested:
Title:
Methods, Challenges, and Promise of Next-Generation Sequencing in Cancer Biology
PMCID:
PMC3238319 -
Hello. I am Huimin, also new to the forum.
Thanks Joann for your suggestion.
A quick question. For RNAseq 50 bp HiSeq2000, v3 chemistry, can I ran 6 samples per lane with single end? or paired end maybe to double the # of reads? My purpose is to get the differential expression between two groups, each group 3 replicates so total 6 samples.
Thanks for your comments!Comment
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Hi
Hi hug2001,
Welcome. For the best exposure of your technical questions to obtain a rapid response here, please be sure to post them by searching/choosing a more specific forum, such as the Illumina forum.Comment
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...06-18-2026, 07:11 AM -
Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...06-02-2026, 10:05 AM
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