Hi! I'm Jackie. I am currently studying Cancer Biology and hope to better my understanding and my knowledge about sequence analysis!
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Welcome
Welcome to the forum, Jackie. Here is a recent open access article I thought I would mention in case you may be interested:
Title:
Methods, Challenges, and Promise of Next-Generation Sequencing in Cancer Biology
PMCID:
PMC3238319 -
Hello. I am Huimin, also new to the forum.
Thanks Joann for your suggestion.
A quick question. For RNAseq 50 bp HiSeq2000, v3 chemistry, can I ran 6 samples per lane with single end? or paired end maybe to double the # of reads? My purpose is to get the differential expression between two groups, each group 3 replicates so total 6 samples.
Thanks for your comments!Comment
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Hi
Hi hug2001,
Welcome. For the best exposure of your technical questions to obtain a rapid response here, please be sure to post them by searching/choosing a more specific forum, such as the Illumina forum.Comment
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...10-30-2026, 06:46 AM
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