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  • BIORELEVANT
    Junior Member
    • Feb 2012
    • 1

    #1

    Hello!

    Hi! I'm Jackie. I am currently studying Cancer Biology and hope to better my understanding and my knowledge about sequence analysis!
  • Joann
    Senior Member
    • Oct 2008
    • 230

    #2
    Welcome

    Welcome to the forum, Jackie. Here is a recent open access article I thought I would mention in case you may be interested:

    Title:
    Methods, Challenges, and Promise of Next-Generation Sequencing in Cancer Biology

    PMCID:
    PMC3238319

    Comment

    • hug2001
      Junior Member
      • Feb 2012
      • 4

      #3
      Hello. I am Huimin, also new to the forum.
      Thanks Joann for your suggestion.
      A quick question. For RNAseq 50 bp HiSeq2000, v3 chemistry, can I ran 6 samples per lane with single end? or paired end maybe to double the # of reads? My purpose is to get the differential expression between two groups, each group 3 replicates so total 6 samples.

      Thanks for your comments!

      Comment

      • Joann
        Senior Member
        • Oct 2008
        • 230

        #4
        Hi

        Hi hug2001,
        Welcome. For the best exposure of your technical questions to obtain a rapid response here, please be sure to post them by searching/choosing a more specific forum, such as the Illumina forum.

        Comment

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