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I am a newbie on NGS. I read a topic on seqanswer.com/wiki/How-to and was confused by the terms transcriptome data vs. genome data. What is the differences between these two types of data? Can anyone help me?
Thank you.
Thank you.
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Hard to say how much biology you know, but here goes: Transcriptome data come from sequencing RNA transcripts of which (generally) the protein coding transcripts are of greatest interest. Genome data comes sequencing the whole genome. If you need more information than then I suggest consulting biology textbooks or even Wikipedia. -
Thanks for reply, Westerman!Comment
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...
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