Hi everyone,
I am Encarnita, french-spanish immunologist working in Japan.
I am using 454 Lifescience Tech. since more than 3 years, but autonomously using 454 Junior since a year. I've been getting many good infos by surfing on that great forum
, but I registered only recently 
!
I've been suffering several troubles since I perform the 454 libraries preparation, emPCR and sequencing by my self, such as too few enriched beads, too much enriched, never-ending washing steps, few passed filter reads out of many raw reads, software upgrade issues leading to new setup after 12 successfull run....
I have no experience on other platforms, but I am eager to learn and I really want to understand precisely what's going on with the 454 steps.
I'll be pleased to help also, if I have any relevant info.
All the best to seqanswers community
!
I am Encarnita, french-spanish immunologist working in Japan.
I am using 454 Lifescience Tech. since more than 3 years, but autonomously using 454 Junior since a year. I've been getting many good infos by surfing on that great forum
, but I registered only recently !I've been suffering several troubles since I perform the 454 libraries preparation, emPCR and sequencing by my self, such as too few enriched beads, too much enriched, never-ending washing steps, few passed filter reads out of many raw reads, software upgrade issues leading to new setup after 12 successfull run....
I have no experience on other platforms, but I am eager to learn and I really want to understand precisely what's going on with the 454 steps.
I'll be pleased to help also, if I have any relevant info.
All the best to seqanswers community
!