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  • ClaudiaLoetsch
    Junior Member
    • Nov 2012
    • 2

    #1

    How to convert bed file to fastq format?

    Hi everyone,
    the data set I want to analyse is only available in .bed format but for galaxy analysis I would need it in Fastq format. Any suggestions would be very appreciated.
    Thanks,
    Claudia
  • dpryan
    Devon Ryan
    • Jul 2011
    • 3478

    #2
    Download bedtools and try the "fastaFromBed" command.

    Just remember that the resulting file may, or may not be useful, since the BED file lacks information about quality scores (thus, why you might as well just make a fasta file rather than fastq) and sequence mismatches (I hope you weren't planning to look for SNPs or anything like that).

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