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  • amarth
    Member
    • Dec 2012
    • 14

    Greetings from a Msc student, Mexico

    Hello Seq-pals!

    I'm a Msc Student, working on Cinvestav, Mexico... My research involves many stuff related to Bioinformatics (or as I can say, my 2nd Hobbie [1st one's Guitar]).

    Despite being spanish-speaker, i can communicate in other languages, like italian, german or english So, i hope the language barrier is not a problem at all...

    I sincerely expect so much on my work, but unfortunately, I'm not sorta Bioinformatics-guru, and I need some advice on you pals, especially on topics related to transcriptome and differential expression (so, i think this is the main reason for joining this forum, lol).

    I wish the best to all of you.
    Sincerely
    Amarth

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  • GATTACAT
    Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by GATTACAT
    Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
    07-01-2026, 11:43 AM
  • SEQadmin2
    Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by SEQadmin2


    I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

    Here are nine questions we think about, in roughly the order they matter, before...
    06-18-2026, 07:11 AM

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