I am a newbie to NGS.We are evaluating the possibility of doing CNV analysis by NGS data eigher by Illumina MiSeq or Ion PGM platform on archived melanoma FFPE sample. We donot want high cycle PCR involved in the protocol which may saturate the result. But both Illumina (targetd enrichment) and Ion PGM protocol (template preparation) include high cycle PCR. Does anyone have a good idea? Thanks.
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CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM
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