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arisa

Junior Member

Join Date: Apr 2013

Posts: 1
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Greetings

04-11-2013, 02:21 AM

Hello people,
I am going to start molecular gut content analysis for Ph.D project. This field and Ion Torrent are new things for me. We are going to use Ion Torrent to analyse my samples from insectivorous bats of Borneo. Glad to find this community.

Thank you,

Cheers,
Arisa
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Nine Things a Sample Prep Scientist Thinks About Before Sequencing

by SEQadmin2

I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

Here are nine questions we think about, in roughly the order they matter, before...
- Channel: Articles
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From Collection to Sequencing: Why Sample Preparation and Preservation Define Sequencing Data

by SEQadmin2

Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.

The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
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- Channel: Articles
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