Has anyone used Blue Pippen to do size selection after adapter ligation. I want to know what size range do you choose. I have done the size selection after the PCR amplification but I want to try to do the size selection before the PCR reaction.
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Pippin Prep help on ChIP-seq applications
Dear Alphy,
I would be happy to try and find a Pippin Prep customer with experience in ChIP-seq, who might be able to help you with those settings. Please send a private message to me (via my SEQanswers profile) with your email address. (I don't want to publish private customer email addresses here.)
Our customers who perform size selection on Truseq libraries prior to amplification enrichment, usually find that they have to use size selection settings significantly larger than the desired insert size (settings 130-150 bp larger than the desired insert sizes, for inserts in the 400-500 bp range).
Chris Boles, Sage Science
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The immune system’s power comes from its genetic diversity, allowing myriad threats to be neutralized through first recognizing foreign antigens. That diversity is also what makes the immune system so difficult to study. Recent advances in sequencing technology and computational biology, however, are giving researchers new tools to understand immune responses and immune-related diseases in greater detail.
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