Hello! - SEQanswers

You are currently viewing the SEQanswers forums as a guest, which limits your access. Click here to register now, and join the discussion

X

tforde

Junior Member

Join Date: Oct 2013

Posts: 7
- Share
- Tweet
#1

Hello!

10-04-2013, 02:38 AM

Hello all,
I am a PhD student at the University of Calgary doing full genome sequence analysis of a bacterial pathogen of wildlife. I'm very new to this area, so thanks in advance for your help getting acquainted with bioinformatics!
Tags: None

Previous template Next

Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing

by GATTACAT

Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
- Channel: Articles
07-01-2026, 11:43 AM
Nine Things a Sample Prep Scientist Thinks About Before Sequencing

by SEQadmin2

I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

Here are nine questions we think about, in roughly the order they matter, before...
- Channel: Articles
06-18-2026, 07:11 AM

	Topics		Statistics	Last Post
	High-Resolution Sequencing Exposes Hidden Toxoplasma Diversity by SEQadmin2 Started by SEQadmin2, 07-02-2026, 11:08 AM		0 responses 25 views 0 reactions	Last Post by SEQadmin2 07-02-2026, 11:08 AM
	New AI Model Captures Long-Range Genomic Signals to Improve RNA Splice Site Prediction by SEQadmin2 Started by SEQadmin2, 06-30-2026, 05:37 AM		0 responses 23 views 0 reactions	Last Post by SEQadmin2 06-30-2026, 05:37 AM
	Large-Scale Protein Screen Uncovers Hidden Regulators of Alternative Polyadenylation by SEQadmin2 Started by SEQadmin2, 06-26-2026, 11:10 AM		0 responses 23 views 0 reactions	Last Post by SEQadmin2 06-26-2026, 11:10 AM
	Whole-Genome Sequencing Traces Faroe Islands Ancestry to a North Atlantic Founder Population by SEQadmin2 Started by SEQadmin2, 06-17-2026, 06:09 AM		0 responses 55 views 0 reactions	Last Post by SEQadmin2 06-17-2026, 06:09 AM

Working...