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  • Marco
    Junior Member
    • Feb 2010
    • 3

    Hello from Michigan

    Hi,

    My name ist Marco and I am working as a postdoc at Michigan State University.
    I am particularly interested in assembling a transcriptome from Solexa and 454 reads and getting expression analysis from the same RNA-seq experiment.
    Unfortunately I am not a computer scientist just a biologist working with a non-model organism but since our bioinformatic specialists are completely overwhelmed with the created data I try to do as many analysis as possible on my own. This is on the one hand exciting (I really start liking the computer work) but also sometimes pretty difficult especially if it comes to tasks which are for most of you pretty simple like parsing an output file. I would really appreciate any help and I am more than happy to share any experience I make on the way.
    Thanks,
    Marco

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  • SEQadmin2
    Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by SEQadmin2


    I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

    Here are nine questions we think about, in roughly the order they matter, before...
    06-18-2026, 07:11 AM
  • SEQadmin2
    From Collection to Sequencing: Why Sample Preparation and Preservation Define Sequencing Data
    by SEQadmin2


    Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.


    The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
    ...
    06-02-2026, 10:05 AM

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