Hello, my name is Alicia and I'm the lab manager of the IPBLN genomics core facility is Spain. We are a public/government lab that provides services on sanger sequencing, NGS, single cell sequencing and spatial transcriptomics. I'm glad to be here and have the opportunity to share with this community our experience and also our problems.
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CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM