Originally posted by sklages
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actually currently I am working on illumina assembly..dont have time for .sff file..because my supervisor want it done first..so when I will start working on .sff, I will let u know...and try everything whatever u have suggested...thanks a lot.. -
@paa6: Before you spend a lot of time on this "assembly" what size genome are you working on? How much data do you have ("x" fold coverage based on the expected genome size)? Unless you have enough data available you may not be able to do a reasonable/sussessful assembly.Comment
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yeah u r right...and since I am new in de novo assembly I dont understand these terms at all...and right now I have to analyse contigs what I have got from illumina....I am slow because I am learning and then sooner after that I have to start 454 assembly...I will take note on ur questions...and will discuss with u sooner...Originally posted by GenoMax View PostComment
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In case you have not discovered these already:
http://res.illumina.com/documents/pr...mbly_ecoli.pdf
http://www.biostars.org/p/52178/Comment
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hey thanks a lot...I have read them though...and I am using geneious software for assembly...Originally posted by GenoMax View PostIn case you have not discovered these already:
http://res.illumina.com/documents/pr...mbly_ecoli.pdf
http://www.biostars.org/p/52178/Comment
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