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Where should i start?
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Spend a half-day reading this posts on this bulletin board. That will give you a good feel for the problems and solutions in NGS as well as questions are frequently asked -- including the "where shall I start" one. :-) -
See if your library has any of the recent books covering NGS, like:
Next-generation Sequencing: Current Technologies and Applications. Jianping Xu (ed.). 2014. Caister Academic PressMichael Black, Ph.D.
ScitoVation LLC. RTP, N.C.Comment
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get some of the 1000 genome data and dick around ........ everything you need to know is on the internet or on this forum
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...06-18-2026, 07:11 AM -
Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...06-02-2026, 10:05 AM
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