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Hi everybody, I am a new user of this forum. I am student in Biotechnology field. I hope I'll get help from this forum.
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Hello and intro
I am working with my colleague on NGS analyses primarily dealing with clinical and environmental prokaryotes. We use the MiSeq and have been experiencing quite the learning curve.
Our location is in the state of Nevada (and yes it is Las Vegas). I look forward to learning much from all and perhaps develop new collaborations.
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...06-18-2026, 07:11 AM -
Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...06-02-2026, 10:05 AM
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