Total RNA Conc. for RNA Seq

[hope2003]

Hello I am a new bee of NextGen Seq. Looking for tips to obtain higher Conc. of Tot RNA from a very small amount of rodent brain tissue to use with RNA Seq kit v2 for Whole Transcriptome-Library Prep. Will greatly appreciate any suggestion!

Thanks!

[sophiespo]

Maybe you can try some RNA-grade glycogen as a carrier during precipitation.

If the RNA quality is low but yield is high you can try re-precipitate.

[williamhorne]

What method are you currently using for RNA isolation on Brian tissue and what is your typical yield? We have done it alot and sequenced it numerous times for customers.

[jwfoley]

There are several published methods for RNA-seq from input as small as a single cell. Don't design your experiment around one kit.

Regarding glycogen as a carrier, consider linear acrylamide instead; glycogen is rumored to inhibit reverse transcription.