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  • vjongene
    Junior Member
    • Apr 2010
    • 1

    Old guy weighing in

    Just discovered this forum from a news item in GenomeWeb. I run a group in Switzerland using GA II and 454 data to analyze genomes and transcriptomes from melanomas. I am now at the National Center for Supercomputing Applications (NCSA) at the University of Illinois, hoping to make NCSA resources and expertise available to the "next-gen" sequencing community.

    I have done quite a bit of high-throughput transcriptome sequence analysis (particularly on the Brazilian ORESTES ESTs and on MPSS data) and been interested for a long time in mapping and assembly problems. I do not code myself, so please do not ask me about how to tweak a Perl script!

    However, I am interested in hearing from anyone who has a BIG problem that is proving intractable (or very difficult) using existing hardware/software platforms. Maybe we can work together.

    Looking forward to meeting people on this forum!

    Cheers,

    Victor J.

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  • SEQadmin2
    Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by SEQadmin2


    I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.


    Here are nine questions we think about, in roughly the order they matter, before...
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  • SEQadmin2
    From Collection to Sequencing: Why Sample Preparation and Preservation Define Sequencing Data
    by SEQadmin2


    Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.


    The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
    ...
    06-02-2026, 10:05 AM

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