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  • rtraborn
    Junior Member
    • Sep 2014
    • 4

    Hello from Bloomington, Indiana

    Hi SEQanswers community:

    I'm a postdoc at Indiana University working on promoter architecture and genome organization in diverse eukaryotes. This site has helped me out on more than one occasion and I figured it was time to officially join and start posting myself.

    I do both experimental and computational work, with a particular emphasis on the latter in recent years.

    My research interests relevant to this site include open-source bioinformatics software, high-performance computing, linux, R programming, data visualization transcriptome analysis and genome annotation.

    I work with multiple type of genomics (and functional genomics) data, especially transcriptome sequence info. I'll post questions as I encounter problems, so that in the future people in similar situations find answers sooner. This looks to be a great group, so I look forward to participating.

    Sincerely,

    Taylor Raborn

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  • GATTACAT
    Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by GATTACAT
    Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
    07-01-2026, 11:43 AM
  • SEQadmin2
    Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by SEQadmin2


    I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

    Here are nine questions we think about, in roughly the order they matter, before...
    06-18-2026, 07:11 AM

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