OTU picking from illumina asssembly of Human Microbiome data

[guptavipin142]

Hi All,

I have 800 gb HQF clean pairend gut illumina data .
i have assembled the data.
Now i want to predict Operational taxonomical Units from assembly fasta contigs.
Apperently none of tool is working well.
What tool I can go with for OTU picking from assemled data sets.
Thanks

[holmrenser]

Maybe you can provide us with a list of tools you tried and why they don't work, since you mentioned you have already tried some!

[guptavipin142]

I have 24 assemblies in fasta format generated from velvet 1.2.10
Tools i have used on assembly
1. DNAclust
2. Cd-hit otu Illumina
3. Ch hit
Finally I am using mOTU. perl script.
Which have wide output.