RNA isolation from sperms

[RAVAL N.]

Hello,
I am getting very less RNA concentration when extracted from RNEasy Mini kit when measured using Qubit but high yield on Nanodrop, and recently I got almost 8 and 9 260/280 ratio but good 260/230 ratio.....i dont know what this much high ratio indicates.....suggestions would be of great help....Thankx in advance...

[dpryan]

In my experience the RNEasy kit doesn't work well at all for sperm RNA extractions. In fact, the only thing we've gotten to work well is Trizol (we're doing mostly small RNAseq on the results and have had good luck with this method). With weird 260/280 ratios like that I wouldn't believe any other value the nanodrop is giving you, they're pretty much guaranteed to be wrong.