Greetings. I'm a graduate student in biomedical and health sciences in Hiroshima University, Japan. My current project is whole genome sequence of Staphylococcus aureus using Illumina MiSeq sequencing platform. I hope we could discuss this matter and we could share many knowledge about microbial genome sequencing.
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...10-30-2026, 06:46 AM
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