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Hi,
I have an environmental sample that contains a mixed microbial population. The sample has been subjected to very harsh conditions, where after I isolated DNA. The idea is to do whole metagenome sequencing on both the environmental sample and after the harsh conditions to compare which communities survived. The problem I have is the DNA concentration is so low after the harsh conditions. I tried the Qubit (with 20 uL), but the concentration was too low to detect. I did PCR on the DNA and I obtained good amplicons for all three domains of life, so I know there is DNA, but not enough for whole metagenome sequencing. What can I do to improve this, without redoing the experiment. (It takes two months to repeat the experiment, and I don't have that time). I was looking at this WGA kit https://www.neb.com/products/e2620-picoplex-wga-kit but this is for single cell. I need something like this for a mixed community from environmental samples. Anyone have ideas please?
I have an environmental sample that contains a mixed microbial population. The sample has been subjected to very harsh conditions, where after I isolated DNA. The idea is to do whole metagenome sequencing on both the environmental sample and after the harsh conditions to compare which communities survived. The problem I have is the DNA concentration is so low after the harsh conditions. I tried the Qubit (with 20 uL), but the concentration was too low to detect. I did PCR on the DNA and I obtained good amplicons for all three domains of life, so I know there is DNA, but not enough for whole metagenome sequencing. What can I do to improve this, without redoing the experiment. (It takes two months to repeat the experiment, and I don't have that time). I was looking at this WGA kit https://www.neb.com/products/e2620-picoplex-wga-kit but this is for single cell. I need something like this for a mixed community from environmental samples. Anyone have ideas please?
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