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  • Khoa.can.Seq
    Junior Member
    • Mar 2015
    • 3

    Hello!

    Hi all. I am new to the NGS community and would like to say hello to everyone!

    I'm looking forward to learning from the community, and to become a contributing member.

    Cheesr,
    Khoa
  • SNPsaurus
    Registered Vendor
    • May 2013
    • 525

    #2
    I think I know you, Khoa, so I'll just say--"Cheesr" back.
    Providing nextRAD genotyping and PacBio sequencing services. http://snpsaurus.com

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    • Khoa.can.Seq
      Junior Member
      • Mar 2015
      • 3

      #3
      Hi "E", yes it's me! I've been reading all your posts on here. Even after all these years, you are still teaching me!
      Last edited by Brian Bushnell; 04-07-2015, 02:40 PM. Reason: Name redacted.

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      • GATTACAT
        Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing
        by GATTACAT
        Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
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      • SEQadmin2
        Nine Things a Sample Prep Scientist Thinks About Before Sequencing
        by SEQadmin2


        I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

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