Hi, a beginner here!
I am trying to run a blast search using a local datbase.
I create the local db using a multifasta file:
makeblastdb -in /root/reference/multiseq.fasta -dbtype 'nucl' -out /root/reference/dbname
this create 3 files in root/reference/dbname: dbname.nhr, .nin and .nsq
then I run:
blastn –db /root/reference/db/dbname –query /root/data/seq.fa –out /root/data/seq_blast_results.out
but I got this error message:Error: Too many positional arguments (1), the offending value: –db
Is the local db is correctly created? could you help?
Thanks
I am trying to run a blast search using a local datbase.
I create the local db using a multifasta file:
makeblastdb -in /root/reference/multiseq.fasta -dbtype 'nucl' -out /root/reference/dbname
this create 3 files in root/reference/dbname: dbname.nhr, .nin and .nsq
then I run:
blastn –db /root/reference/db/dbname –query /root/data/seq.fa –out /root/data/seq_blast_results.out
but I got this error message:Error: Too many positional arguments (1), the offending value: –db
Is the local db is correctly created? could you help?
Thanks
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