Blastn error: positional arguments ?

[bpich]

Hi, a beginner here!

I am trying to run a blast search using a local datbase.

I create the local db using a multifasta file:
makeblastdb -in /root/reference/multiseq.fasta -dbtype 'nucl' -out /root/reference/dbname
this create 3 files in root/reference/dbname: dbname.nhr, .nin and .nsq

then I run:
blastn –db /root/reference/db/dbname –query /root/data/seq.fa –out /root/data/seq_blast_results.out

but I got this error message:Error: Too many positional arguments (1), the offending value: –db

Is the local db is correctly created? could you help?

Thanks

[dschika]

...this create 3 files in root/reference/dbname: dbname.nhr, .nin and .nsq

then I run:
blastn –db /root/reference/db/dbname –query /root/data/seq.fa –out /root/data/seq_blast_results.out

root/reference/dbname vs. /root/reference/db/dbname

May that be your problem?

What happens if you just type:

-db dbname

[bpich]

my mistake! the cmd is
blastn –db /root/reference/dbname –query /root/data/seq.fa –out /root/data/seq_blast_results.out

[GenoMax]

Try the solution recommended by A. Mstt in this thread: http://stackoverflow.com/questions/2...ending-value-f

[bpich]

Many thanks. work perfectly now