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I am a newcomer to Bioinformatics and I mojor in complex polygenic disorder, especially Cardiovascular disease and Type II Diabetes. Now, I pay attention to Orthopaedics disease. Actually, to me, this is a brand-new domain. Ask you master, how to conduct Precision Medicine in Surgical Diseases, especially in Orthopaedics disease. What kind of disease could be considered as a research field? For instance, degenerative disease or trauma orthopaedics, congenital diseases or malformation, in other words, complex polygenic disorder or monogenic disease? Now I am interesting in comparative genomic hybridization, quantitative polymerase-chainreaction analysis, and DNA sequencing, it's important to consider that the direction of research on Orthopaedics disease.
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Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.07-01-2026, 11:43 AM
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...