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molbionerd

Junior Member

Join Date: May 2016

Posts: 3
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Hi

06-03-2016, 11:12 AM

Just wanted to introduce myself, as that is what the site told me to do.

I am a user of Ion Torrent PGM and Illumina Miseq currently in the Genomics Facility. I have just started as the facility director. I have a lot of experience with RNAseq on the PGM as well as some microbiome (16s) work. I have used the PGM to do characterization of mutants and what not (mostly prokaryotic because as we know the PGM doesn't go very deep) and for transcript discovery/genome re-annotation projects.

New to Illumina. I am hoping to start my first Illumina RNAseq on Monday and have been using this site to figure out what the hell I'm doing. I also have several projects in the pipeline for microbiome, ChIP-seq, exosome sequencing and some targeted RNAseq and RNA structure seqs.

I have a decent amount of experience with general molecular techniques and lab procedures (again mostly in prokaryotes as this was my training). Always willing to help anyone I can and always trying to learn something new.
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

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