split peak in RNA-seq libarary

[Ikay]

Hi,

I am new to RNA-seq library preparation. I made some libraries with NuGEN Ovation RNA-seq kit for Arabidopsis. The library was made with rice leaf RNA. The bioanalyzer trace of the final library show a split peak (rather than a smooth, bell shaped one generally seen). Is it usable for sequencing? If you guys have any suggestions for improvement for me, I will appreciate that. The trace is attached.

Thank you
IKay

[nucacidhunter]

Library can be sequenced. Peaks could be due to inefficient InDA-C mediated depletion of rRNA which can be removed bioinformatically. NuGEN Tech Support should be able to help.