Hi, does anyone experienced after a SureSelect QXT libraryprep workflow and Miseq run an output of really good reads in forward direction and absolutely bad quality scores for the reads in reverse direction? Thanks in advance
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For reference cross-posted: https://www.biostars.org/p/220245/
It is ok to cross-post on SeqAnswers.
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CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM
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