Hello everyone. New to NGS. I have run small RNA run on Miseq using Truseq small rna library preparation kit from Illumina. Got very low clusters 153 k/mm2. the final library concentration as measured with qubit ranged from 5-20nM & final used library was 10pM. Kindly give the inputs for the same.
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CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM