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I am a graduate student in the Chinese University of Hong Kong. 
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Marco -
Hi, I am a graduate student in the University of Hong Kong.
Are you still working in NGS? -
Nice to meet you Y Y Leung.
Yes. You can contact me for more.MarcoComment
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Hi Marco,
What specific area of NGS are you working in? and also may i ask what dept you are from?
I am in the engineering side.
Regards,
Y.Y.Comment
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Dear Y Y Leung,
Biochemistry. I am doing research on human genome using Solexa. I suppose you are working on algorithm in read mapping? or similar things?MarcoComment
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Dear Marco,
I am working on data analysis mainly. I am just a new starter. But I guess I would be working on RNA-seq and targetted sequencing these two areas.
I think I would be playing with algorithms. What software are you using?
FannyComment
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...06-18-2026, 07:11 AM -
Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...06-02-2026, 10:05 AM
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