Hi everyone, I'm a post-doc at the University of Utah. I've been working on some transcriptome analysis using paired GAII seq reads in platelets. The posts I've seen here have been very helpful. I'm excited to begin contributing.
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CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM